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pft β hydrobromide  (MedChemExpress)


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    MedChemExpress pft β hydrobromide
    Fig. 7 SQLE increases tumor cell proliferation and inhibits cell apoptosis of Bca by regulating the PTEN/AKT/GSK3β signaling pathway through P53. (A) Protein expression levels of P53, PTEN, AKT, P-AKT, GSK3β, P-GSK3β, Bcl2, and Cyclin D1 in T24 and 5637 cells; (B, C) The CCK-8 assay was used to assess the proliferation of shSQLE-treated tumor cells, NC-treated tumor cells, negative controls, and cells treated with shSQLE + <t>PFT</t> <t>β</t> from 0 to 96 h; (D) Rep resentative images of the colony-forming assay; (E, F) Representative images of KI67 immunostaining showed that overexpression of P53 increased the proliferation of T24 and 5637 cells after SQLE knockdown; (G–I) Flow cytometry detected the early apoptosis and late apoptosis cell population in T24 and 5637. Data represent the mean ± SD of three independent experiments (*p < 0.05, **p < 0.01, ***p < 0.001, ns, not significant)
    Pft β Hydrobromide, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 23 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pft+%CE%B2+hydrobromide/Pifithrin-%CE%B2+hydrobromide/pm37770925-54-0-16
    Average 94 stars, based on 23 article reviews
    pft β hydrobromide - by Bioz Stars, 2026-09
    94/100 stars

    Images

    1) Product Images from "SQLE Knockdown inhibits bladder cancer progression by regulating the PTEN/AKT/GSK3β signaling pathway through P53."

    Article Title: SQLE Knockdown inhibits bladder cancer progression by regulating the PTEN/AKT/GSK3β signaling pathway through P53.

    Journal: Cancer cell international

    doi: 10.1186/s12935-023-02997-5

    Fig. 7 SQLE increases tumor cell proliferation and inhibits cell apoptosis of Bca by regulating the PTEN/AKT/GSK3β signaling pathway through P53. (A) Protein expression levels of P53, PTEN, AKT, P-AKT, GSK3β, P-GSK3β, Bcl2, and Cyclin D1 in T24 and 5637 cells; (B, C) The CCK-8 assay was used to assess the proliferation of shSQLE-treated tumor cells, NC-treated tumor cells, negative controls, and cells treated with shSQLE + PFT β from 0 to 96 h; (D) Rep resentative images of the colony-forming assay; (E, F) Representative images of KI67 immunostaining showed that overexpression of P53 increased the proliferation of T24 and 5637 cells after SQLE knockdown; (G–I) Flow cytometry detected the early apoptosis and late apoptosis cell population in T24 and 5637. Data represent the mean ± SD of three independent experiments (*p < 0.05, **p < 0.01, ***p < 0.001, ns, not significant)
    Figure Legend Snippet: Fig. 7 SQLE increases tumor cell proliferation and inhibits cell apoptosis of Bca by regulating the PTEN/AKT/GSK3β signaling pathway through P53. (A) Protein expression levels of P53, PTEN, AKT, P-AKT, GSK3β, P-GSK3β, Bcl2, and Cyclin D1 in T24 and 5637 cells; (B, C) The CCK-8 assay was used to assess the proliferation of shSQLE-treated tumor cells, NC-treated tumor cells, negative controls, and cells treated with shSQLE + PFT β from 0 to 96 h; (D) Rep resentative images of the colony-forming assay; (E, F) Representative images of KI67 immunostaining showed that overexpression of P53 increased the proliferation of T24 and 5637 cells after SQLE knockdown; (G–I) Flow cytometry detected the early apoptosis and late apoptosis cell population in T24 and 5637. Data represent the mean ± SD of three independent experiments (*p < 0.05, **p < 0.01, ***p < 0.001, ns, not significant)

    Techniques Used: Expressing, CCK-8 Assay, Immunostaining, Over Expression, Knockdown, Flow Cytometry

    Related Articles

    Concentration Assay:

    Article Title: SQLE Knockdown inhibits bladder cancer progression by regulating the PTEN/AKT/GSK3β signaling pathway through P53.
    Article Snippet: .. PFT-β hydrobromide (purity of 99.97%, PFT-β), an inhibitor of the p53 protein [19], was purchased from MedChemExpress (Shanghai, China) and dissolved in dimethyl sulfoxide (DMSO) to a certain concentration. .. The following antibodies were used in the present study: SQLE (TD12063, Abmart), KI67 (27309-1-AP, Prointech), P-AKT (66444-1-Ig, Proteintech), AKT (60203- 2-Ig, Proteintech), P-GSK-3β (#9323, CST), GSK-3β (#9832, CST), PTEN (60300-1-Ig, Proteintech), P53 (ab26, Abcam), Cyclin D1 (26939-1-AP, Proteintech), Bcl-2 (#15,071, CST), Bax (60267-1-Ig, Proteintech), cleaved-Caspase-3 (#9664, CST), and GAPDH (60004-1- Ig, Proteintech).



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    Fig. 7 SQLE increases tumor cell proliferation and inhibits cell apoptosis of Bca by regulating the PTEN/AKT/GSK3β signaling pathway through P53. (A) Protein expression levels of P53, PTEN, AKT, P-AKT, GSK3β, P-GSK3β, Bcl2, and Cyclin D1 in T24 and 5637 cells; (B, C) The CCK-8 assay was used to assess the proliferation of shSQLE-treated tumor cells, NC-treated tumor cells, negative controls, and cells treated with shSQLE + <t>PFT</t> <t>β</t> from 0 to 96 h; (D) Rep resentative images of the colony-forming assay; (E, F) Representative images of KI67 immunostaining showed that overexpression of P53 increased the proliferation of T24 and 5637 cells after SQLE knockdown; (G–I) Flow cytometry detected the early apoptosis and late apoptosis cell population in T24 and 5637. Data represent the mean ± SD of three independent experiments (*p < 0.05, **p < 0.01, ***p < 0.001, ns, not significant)
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    Image Search Results


    Fig. 7 SQLE increases tumor cell proliferation and inhibits cell apoptosis of Bca by regulating the PTEN/AKT/GSK3β signaling pathway through P53. (A) Protein expression levels of P53, PTEN, AKT, P-AKT, GSK3β, P-GSK3β, Bcl2, and Cyclin D1 in T24 and 5637 cells; (B, C) The CCK-8 assay was used to assess the proliferation of shSQLE-treated tumor cells, NC-treated tumor cells, negative controls, and cells treated with shSQLE + PFT β from 0 to 96 h; (D) Rep resentative images of the colony-forming assay; (E, F) Representative images of KI67 immunostaining showed that overexpression of P53 increased the proliferation of T24 and 5637 cells after SQLE knockdown; (G–I) Flow cytometry detected the early apoptosis and late apoptosis cell population in T24 and 5637. Data represent the mean ± SD of three independent experiments (*p < 0.05, **p < 0.01, ***p < 0.001, ns, not significant)

    Journal: Cancer cell international

    Article Title: SQLE Knockdown inhibits bladder cancer progression by regulating the PTEN/AKT/GSK3β signaling pathway through P53.

    doi: 10.1186/s12935-023-02997-5

    Figure Lengend Snippet: Fig. 7 SQLE increases tumor cell proliferation and inhibits cell apoptosis of Bca by regulating the PTEN/AKT/GSK3β signaling pathway through P53. (A) Protein expression levels of P53, PTEN, AKT, P-AKT, GSK3β, P-GSK3β, Bcl2, and Cyclin D1 in T24 and 5637 cells; (B, C) The CCK-8 assay was used to assess the proliferation of shSQLE-treated tumor cells, NC-treated tumor cells, negative controls, and cells treated with shSQLE + PFT β from 0 to 96 h; (D) Rep resentative images of the colony-forming assay; (E, F) Representative images of KI67 immunostaining showed that overexpression of P53 increased the proliferation of T24 and 5637 cells after SQLE knockdown; (G–I) Flow cytometry detected the early apoptosis and late apoptosis cell population in T24 and 5637. Data represent the mean ± SD of three independent experiments (*p < 0.05, **p < 0.01, ***p < 0.001, ns, not significant)

    Article Snippet: PFT-β hydrobromide (purity of 99.97%, PFT-β), an inhibitor of the p53 protein [19], was purchased from MedChemExpress (Shanghai, China) and dissolved in dimethyl sulfoxide (DMSO) to a certain concentration.

    Techniques: Expressing, CCK-8 Assay, Immunostaining, Over Expression, Knockdown, Flow Cytometry